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AlexaFluor594,即AF594，是一种水溶性的红荧光染料，是著名的AF系列染料主要成员之一。AF594荧光和Cy3.5类似，但是性质非常稳定，长时间曝光不会光漂白，荧光也非常亮，发光效率很高，并且荧光在pH4-10范围内不受影响。AF594是磺化的荧光染料，水溶性很好，标记蛋白不会引起蛋白聚集，偶联小分子能增强小分子水溶性，是一种性能很高端的荧光标记染料。
NHS活化酯(N-羟基琥珀酰亚胺酯，N-hydroxysuccinimideester，succinimidylester,SE)是生物标记反应中最常用的活化基团。NHS活化AF594分子中的羧基，让它可以和目标生物分子上的胺基（主要伯胺）反应生成稳定的酰胺键，从而把AF594荧光分子标记到生物大分子上。
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德尔塔生物其他荧光染料：
AlexaFluor350
AlexaFluor405
AlexaFluor488
AlexaFluor532
AlexaFluor546
AlexaFluor555
AlexaFluor561
AlexaFluor568
AlexaFluor594
AlexaFluor647
AlexaFluor660
AlexaFluor680
AlexaFluor700
AlexaFluor750
BODIPYFL
Coumarin
Cy3
Cy5
Fluorescein(FITC)
OregonGreen
PacificBlue
PacificGreen
PacificOrange
PE-Cyanine7
PerCP-Cyanine5.5
Tetramethylrhodamine(TRITC)
TexasRed
SuperBright和eFluor染料
eFluor450
eFluor506
eFluor660
PEeFluor610
PerCP-eFluor710
APC-eFluor780
SuperBright436
SuperBright600
SuperBright645
SuperBright702
SuperBright780
DNA染料
DAPI
PropidiumIodide
SYTO9
SYTOXGreen
TO-PRO-3
Qdot探针
Qdot525
Qdot565
Qdot605
Qdot655
Qdot705
Qdot800
荧光蛋白标记
Allophycocyanin(APC)
R-Phycoerythrin(R-PE)
表达的荧光蛋白
CyanFluorescentProtein(CFP)
GreenFluorescentProtein(GFP)
RedFluorescentProtein(RFP)
荧光染料选择
FluorophoreSelectionOverview
BUV染料
BrilliantUltraVioletPolymerDyes
BrilliantUltraViolet395
BrilliantUltraViolet496
BrilliantUltraViolet563
BrilliantUltraViolet615
BrilliantUltraViolet661
BrilliantUltraViolet737
BrilliantUltraViolet805
BrilliantViolet421
BrilliantViolet480
BrilliantViolet650
BrilliantViolet711
BrilliantViolet786
NovaFluor染料
NovaFluorBlue510Dye
NovaFluorBlue530Dye
NovaFlurBlue555Dye
NovaFluorBlue585Dye
NovaFluorBlue610-30SDye
NovaFluorBlue610-70SDye
NovaFluorBlue660-40SDye
NovaFluorBlue660-120SDye
NovaFluorYellow570Dye
NovaFluorYellow590Dye
NovaFluorYellow610Dye
NovaFluorYellow660Dye
NovaFluorYellow690Dye
NovaFluorYellow700Dye
NovaFluorYellow730Dye
NovaFluorRed660Dye
NovaFluorRed685Dye
NovaFluorRed700Dye
NovaFluorRed710Dye
以上信息由德尔塔生物为您提供!