Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. H2DCFDA is dissolved in DMSO to obtain a 10 mM stock solutions and further diluted before use.
2. Cells are incubated with 5 µM H2DCFDA solution in PBS in the dark for 30 min at 37°C, then harvested with 0.05% trypsin-EDTA solution, suspended in a fresh medium, and immediately analyzed with flow cytometer.
3. Along with the H2DCFDA probe, if indicated, use ROS-insensitive modification of the fluorescein dye DCFDA as a positive control. The staining procedure is the same as for the H2DCFDA^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

487.29

Solid

C₂₄H₁₆Cl₂O₇

4091-99-0

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

In Vitro: 

DMSO : 125 mg/mL (256.52 mM; Need ultrasonic)

Ethanol : 20 mg/mL (41.04 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量1 mg5 mg10 mg1 mM2.0522 mL10.2608 mL20.5217 mL5 mM0.4104 mL2.0522 mL4.1043 mL10 mM0.2052 mL1.0261 mL2.0522 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。