FAM azide| FAM N3|FAM叠氮化物
FAM azide for Click chemistry labeling. FAM remains one of the most popular fluorescent lablels for various application. Most instruments capable of fluorescence detection, ranging from plate readers to fluorescence microscopes, are able to work in FAM channel.
With versatility of Click chemistry and this reagent, it is possible to attach this popular fluorophore to nearly any alkyne bearing molecule.
FAM azide is available both as solid compound, and as 10 mM solution in DMSO ready to use in our recommended labeling protocol. This product is a pure 5-isomer. FAM is a replacement for Alexa Fluor 488, DyLight 488.
FAM azide| FAM N3|FAM叠氮化物
 
General properties：
Appearance:
yellowish crystals
Molecular weight:
458.42
Molecular formula:
C24H18N4O6
Solubility:
soluble in polar organic solvents (DMF, DMSO, alcohols)
Quality control:
NMR 1H, HPLC-MS (95%)
Storage conditions:
Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
Spectral properties：
Excitation maximum, nm:
494
ε, L⋅mol−1⋅cm−1:
75000
Emission maximum, nm:
520
Fluorescence quantum yield:
0.9
CF260:
0.20
CF280:
0.17
荧光染料示例
激发光
Indo-1, Ca saturated
331 nm
Indo-1 Ca2+
346 nm
Cascade Blue BSA pH 7.0
401 nm
Cascade Blue
398 nm
LysoTracker Blue
373 nm
Alexa 405
401 nm
LysoSensor Blue pH 5.0
374 nm
LysoSensor Blue
374 nm
DyLight 405
399 nm
DyLight 350
332 nm
BFP (Blue Fluorescent Protein)
380 nm
Alexa 350
343 nm
7-Amino-4-methylcoumarin pH 7.0
346 nm
Amino Coumarin
345 nm
AMCA conjugate
347 nm
Coumarin
360 nm
7-Hydroxy-4-methylcoumarin
360 nm
7-Hydroxy-4-methylcoumarin pH 9.0
361 nm
6,8-Difluoro-7-hydroxy-4-methylcoumarin pH 9.0
358 nm
Hoechst 33342
352 nm
Pacific Blue
404 nm
Hoechst 33258
352 nm
Hoechst 33258-DNA
352 nm
Pacific Blue antibody conjugate pH 8.0
404 nm
PO-PRO-1
434 nm
PO-PRO-1-DNA
435 nm
POPO-1
433 nm
POPO-1-DNA
433 nm
DAPI-DNA
359 nm
DAPI
358 nm
Marina Blue
362 nm
SYTOX Blue-DNA
445 nm
CFP (Cyan Fluorescent Protein)
434 nm
eCFP (Enhanced Cyan Fluorescent Protein)
437 nm
1-Anilinonaphthalene-8-sulfonic acid (1,8-ANS)
375 nm
Indo-1, Ca free
346 nm
1,8-ANS (1-Anilinonaphthalene-8-sulfonic acid)
375 nm
BO-PRO-1-DNA
462 nm
BOPRO-1
462 nm
BOBO-1-DNA
461 nm
SYTO 45-DNA
451 nm
evoglow-Pp1
448 nm
evoglow-Bs1
448 nm
evoglow-Bs2
448 nm
Auramine O
431 nm
DiO
487 nm
LysoSensor Green pH 5.0
447 nm
Cy 2
489 nm
LysoSensor Green
447 nm
Fura-2, high Ca
336 nm
Fura-2 Ca2+sup>
336 nm
SYTO 13-DNA
488 nm
YO-PRO-1-DNA
491 nm
YOYO-1-DNA
491 nm
eGFP (Enhanced Green Fluorescent Protein)
488 nm
LysoTracker Green
503 nm
GFP (S65T)
489 nm
BODIPY FL, MeOH
502 nm
Sapphire
396 nm
BODIPY FL conjugate
503 nm
MitoTracker Green
490 nm
MitoTracker Green FM, MeOH
490 nm
Fluorescein 0.1 M NaOH
493 nm
Calcein pH 9.0
494 nm
Fluorescein pH 9.0
490 nm
Calcein
493 nm
Fura-2, no Ca
367 nm
Fluo-4
494 nm
FDA
495 nm
DTAF
495 nm
Fluorescein
495 nm
Fluorescein antibody conjugate pH 8.0
493 nm
CFDA
495 nm
FITC
495 nm
Alexa Fluor 488 hydrazide-water
493 nm
DyLight 488
493 nm
5-FAM pH 9.0
492 nm
FITC antibody conjugate pH 8.0
495 nm
Alexa 488
493 nm
Rhodamine 110
497 nm
Rhodamine 110 pH 7.0
497 nm
Acridine Orange
431 nm
Alexa Fluor 488 antibody conjugate pH 8.0
499 nm
BCECF pH 5.5
485 nm
PicoGreendsDNA quantitation reagent
502 nm
SYBR Green I
498 nm
Rhodaminen Green pH 7.0
497 nm
CyQUANT GR-DNA
502 nm
NeuroTrace 500/525, green fluorescent Nissl stain-RNA
497 nm
DansylCadaverine
335 nm
Rhodol Green antibody conjugate pH 8.0
499 nm
Fluoro-Emerald
495 nm
Nissl
497 nm
Fluorescein dextran pH 8.0
501 nm
Rhodamine Green
497 nm
5-(and-6)-Carboxy-2', 7'-dichlorofluorescein pH 9.0
504 nm
DansylCadaverine, MeOH
335 nm
eYFP (Enhanced Yellow Fluorescent Protein)
514 nm
Oregon Green 488
498 nm
Oregon Green 488 antibody conjugate pH 8.0
498 nm
Fluo-3
506 nm
BCECF pH 9.0
501 nm
SBFI-Na+
336 nm
Fluo-3 Ca2+
506 nm
Rhodamine 123, MeOH
507 nm
FlAsH
509 nm
Calcium Green-1 Ca2+
506 nm
Magnesium Green
507 nm
DM-NERF pH 4.0
493 nm
Calcium Green
506 nm
Citrine
515 nm
LysoSensor Yellow pH 9.0
335 nm
TO-PRO-1-DNA
515 nm
Magnesium Green Mg2+
507 nm
Sodium Green Na+
507 nm
TOTO-1-DNA
514 nm
Oregon Green 514
512 nm
Oregon Green 514 antibody conjugate pH 8.0
513 nm
NBD-X
466 nm
DM-NERF pH 7.0
509 nm
NBD-X, MeOH
467 nm
CI-NERF pH 6.0
513 nm
Alexa 430
431 nm
Alexa Fluor 430 antibody conjugate pH 7.2
431 nm
CI-NERF pH 2.5
504 nm
Lucifer Yellow, CH
428 nm
LysoSensor Yellow pH 3.0
389 nm
6-TET, SE pH 9.0
521 nm
Eosin antibody conjugate pH 8.0
525 nm
Eosin
524 nm
6-Carboxyrhodamine 6G pH 7.0
526 nm
6-Carboxyrhodamine 6G, hydrochloride
525 nm
Bodipy R6G SE
528 nm
BODIPY R6G, MeOH
528 nm
6 JOE
520 nm
Cascade Yellow antibody conjugate pH 8.0
399 nm
Cascade Yellow
399 nm
mBanana
540 nm
Alexa Fluor 532 antibody conjugate pH 7.2
528 nm
Alexa 532
528 nm
Erythrosin-5-isothiocyanate pH 9.0
533 nm
6-HEX, SE pH 9.0
534 nm
mOrange
548 nm
mHoneydew
478 nm
Cy 3
549 nm
Rhodamine B
543 nm
DiI
551 nm
5-TAMRA-MeOH
543 nm
Alexa 555
553 nm
Alexa Fluor 555 antibody conjugate pH 7.2
553 nm
DyLight 549
555 nm
BODIPY TMR-X, SE
544 nm
BODIPY TMR-X, MeOH
544 nm
PO-PRO-3-DNA
539 nm
PO-PRO-3
539 nm
Rhodamine
551 nm
Bodipy TMR-X conjugate
544 nm
POPO-3
533 nm
Alexa 546
562 nm
BODIPY TMR-X antibody conjugate pH 7.2
544 nm
Calcium Orange Ca2+
549 nm
TRITC
550 nm
Calcium Orange
549 nm
Rhodaminephalloidin pH 7.0
558 nm
MitoTracker Orange
551 nm
MitoTracker Orange, MeOH
551 nm
Phycoerythrin
565 nm
Magnesium Orange
550 nm
R-Phycoerythrin pH 7.5
565 nm
5-TAMRA pH 7.0
553 nm
5-TAMRA
549 nm
Rhod-2
552 nm
FM 1-43
472 nm
Rhod-2 Ca2+
553 nm
Tetramethylrhodamine antibody conjugate pH 8.0
552 nm
FM 1-43 lipid
473 nm
LOLO-1-DNA
568 nm
dTomato
554 nm
DsRed
563 nm
Dapoxyl (2-aminoethyl) sulfonamide
372 nm
Tetramethylrhodamine dextran pH 7.0
555 nm
Fluor-Ruby
554 nm
Resorufin
571 nm
Resorufin pH 9.0
571 nm
mTangerine
568 nm
LysoTracker Red
578 nm
Lissaminerhodamine
572 nm
Cy 3.5
578 nm
Rhodamine Red-X antibody conjugate pH 8.0
573 nm
Sulforhodamine 101, EtOH
578 nm
JC-1 pH 8.2
593 nm
JC-1
592 nm
mStrawberry
575 nm
MitoTracker Red
578 nm
MitoTracker Red, MeOH
578 nm
X-Rhod-1 Ca2+
580 nm
Alexa Fluor 568 antibody conjugate pH 7.2
579 nm
Alexa 568
576 nm
5-ROX pH 7.0
578 nm
5-ROX (5-Carboxy-X-rhodamine, triethylammonium salt)
578 nm
BO-PRO-3-DNA
574 nm
BOPRO-3
574 nm
BOBO-3-DNA
570 nm
Ethidium Bromide
524 nm
ReAsH
597 nm
Calcium Crimson
589 nm
Calcium Crimson Ca2+
590 nm
mRFP
585 nm
mCherry
587 nm
Texas Red-X antibody conjugate pH 7.2
596 nm
HcRed
590 nm
DyLight 594
592 nm
Ethidium homodimer-1-DNA
528 nm
Ethidiumhomodimer
528 nm
Propidium Iodide
538 nm
SYPRO Ruby
467 nm
Propidium Iodide-DNA
538 nm
Alexa 594
590 nm
BODIPY TR-X, SE
588 nm
BODIPY TR-X, MeOH
588 nm
BODIPY TR-X phallacidin pH 7.0
590 nm
Alexa Fluor 610 R-phycoerythrin streptavidin pH 7.2
567 nm
YO-PRO-3-DNA
613 nm
Di-8 ANEPPS
469 nm
Di-8-ANEPPS-lipid
469 nm
YOYO-3-DNA
612 nm
Nile Red-lipid
553 nm
Nile Red
559 nm
DyLight 633
624 nm
mPlum
587 nm
TO-PRO-3-DNA
642 nm
DDAO pH 9.0
648 nm
Fura Red, high Ca
434 nm
Allophycocyanin pH 7.5
651 nm
APC (allophycocyanin)
650 nm
Nile Blue, EtOH
631 nm
TOTO-3-DNA
642 nm
Cy 5
646 nm
BODIPY 650/665-X, MeOH
646 nm
Alexa Fluor 647 R-phycoerythrin streptavidin pH 7.2
569 nm
DyLight 649
652 nm
Alexa Fluor 647 antibody conjugate pH 7.2
653 nm
Alexa 647
653 nm
Fura Red Ca2+
435 nm
Atto 647
644 nm
Fura Red, low Ca
472 nm
Carboxynaphthofluorescein pH 10.0
600 nm
Alexa 660
664 nm
Alexa Fluor 660 antibody conjugate pH 7.2
663 nm
Cy 5.5
673 nm
Alexa Fluor 680 antibody conjugate pH 7.2
679 nm
Alexa 680
679 nm
DyLight 680
678 nm
Alexa Fluor 700 antibody conjugate pH 7.2
696 nm
Alexa 700
696 nm
FM 4-64, 2% CHAPS
506 nm
FM 4-64
508 nm